Methods Study the action potential and influx calcium ion current of ventricular muscle cells of guinea pigs by using Langendorff model and patch-clamp technique. 方法利用Langendorff模型以及膜片钳技术研究心肌细胞内向钙电流。
Methods To isolate human mesenteric artery smooth muscle cells with acute enzyme separation method and record the electric current of the calcium-activated potassium channels in these cells using patch clamp single channel technique. 方法用急性酶分离法分离人肠系膜平滑肌细胞,采用单通道膜片钳技术记录该细胞上的钙激活钾通道电流。
Record the influence of BK in single smooth muscle cell by patch clamp amplifer. 分离平滑肌细胞用膜片钳记录钙通道电流,观察BK对单个平滑肌细胞的影响。
In the present work the mechanism of the effect of cGMP on KCa channel in primary cultured porcine coronary artery smooth muscle cells using patch clamp technique was investigated. 本工作应用膜片箝技术以原代培养猪冠状动脉平滑肌细胞为对象研究了cGMP影响KCa通道的机制。
Methods Papain, collagenase and hyaluronidase were used to isolate single human mesenteric arteriolar smooth muscle cells and whole-cell recording techniques of patch clamp was used to record channel current. 方法采用木瓜蛋白酶、胶原酶和透明质酸酶分离出人肠系膜小动脉平滑肌细胞,用膜片钳全细胞记录法记录通道电流。
Methods Action potential of papillary muscle was studied with standard microelectrode technique and the ion currents of single ventricular myocyte in guinea pig were measured by whole cell patch clamp technique. 方法用标准微电极技术和全细胞膜片钳技术,分别测定豚鼠乳头状肌AP和单个心室肌细胞离子电流。
Whole-cell patch clamping: Whole cell currents were recorded in freshly isolated rabbit mesentery artery smooth muscle cells via the whole-cell patch clamp technique. 全细胞膜片钳(whole-cellpatchclamp)技术:胶原酶法新鲜分离单个家兔肠系膜阻力血管平滑肌细胞,全细胞电压钳制状态下监测应用目的药物Oxy前后外向钾电流&钙敏感钾通道的电流变化。
The action of chuanxiong extract on Ca2+-activated K channel ( Kca) of smooth muscle of coronary artery in pigs was studied by patch clamp technique. 用膜片钳方法研究川芎提取液对猪冠状动脉平滑肌钙激活钾通道的作用。
Using enzymatic isolation method, we get a number of primary thoracic aortic smooth muscle cells of SD rats for patch clamp technique. 采用酶消化法为膜片钳实验提供大量SD大鼠原代胸主动脉平滑肌细胞。